automated dapi nuclei counting Search Results


dapi  (FUJIFILM)
90
FUJIFILM dapi
Dapi, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automated+dapi+nuclei+counting/dapi/pmc05226944-47-6-9
Average 90 stars, based on 1 article reviews
dapi - by Bioz Stars, 2026-09
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4’,6-diamidino-2-phenylindole  (FUJIFILM)
90
FUJIFILM 4’,6-diamidino-2-phenylindole
Changes in HMGB1 levels and localization in response to neutron irradiation with BPA in SAS cell-derived tumors. SAS cells were subcutaneously grafted in nude mice and 30 min after administration of BPA–fructose at 500 mg/kg bodyweight, tumors were mock-irradiated ( E ) or locally irradiated for 60 min with neutron beam, (( F ), BNCT). ( A ) Changes in tumor volumes in response to neutron irradiation after BPA administration. Data are presented as mean ± S.E. *, p < 0.05. ( B ) Measurement of plasma levels of human HMGB1 in mice using ELISA (Abnova). ( C ) Mouse plasma HMGB1 levels of C57BL/6 mice without tumor xenograft 3 days after mock irradiation or BNCT measured using ELISA kit (Novus Biologicals). The BNCT group was administered with BPA–fructose at 500 mg/kg bodyweight 30 min before whole-body neutron irradiation. ( D ) Immunostaining of 53BP1 in sections from tumor xenografts of ( A ) at day 3. Bars in ( D ), 50 µm. ( E , F ) Immunostaining of the HMGB1 (green) and β-actin (red) in sections from tumor xenograft-bearing mice at day 3. Bars, 200 µm (top, left panel) and 50 µm (other panels). In ( F ), HMGB1 panel, solid red arrow shows the distribution of HMGB1 in the cytoplasm; solid white arrow shows the irregular nuclear morphology. Day 3: mock irradiation, n = 7; BNCT group, n = 7. Day 8: mock irradiation, n = 4; BNCT group, n = 4. Physical dose at the skin was estimated to be 6.5 Gy in the BNCT group. For counterstaining <t>of</t> <t>nuclei,</t> <t>4′,6-diamidino-2-phenylindole</t> (blue) was used.
4’,6 Diamidino 2 Phenylindole, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/automated+dapi+nuclei+counting/4++6+diamidino+2+phenylindole++dapi/pmc08945761-51-16-22
Average 90 stars, based on 1 article reviews
4’,6-diamidino-2-phenylindole - by Bioz Stars, 2026-09
90/100 stars
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Changes in HMGB1 levels and localization in response to neutron irradiation with BPA in SAS cell-derived tumors. SAS cells were subcutaneously grafted in nude mice and 30 min after administration of BPA–fructose at 500 mg/kg bodyweight, tumors were mock-irradiated ( E ) or locally irradiated for 60 min with neutron beam, (( F ), BNCT). ( A ) Changes in tumor volumes in response to neutron irradiation after BPA administration. Data are presented as mean ± S.E. *, p < 0.05. ( B ) Measurement of plasma levels of human HMGB1 in mice using ELISA (Abnova). ( C ) Mouse plasma HMGB1 levels of C57BL/6 mice without tumor xenograft 3 days after mock irradiation or BNCT measured using ELISA kit (Novus Biologicals). The BNCT group was administered with BPA–fructose at 500 mg/kg bodyweight 30 min before whole-body neutron irradiation. ( D ) Immunostaining of 53BP1 in sections from tumor xenografts of ( A ) at day 3. Bars in ( D ), 50 µm. ( E , F ) Immunostaining of the HMGB1 (green) and β-actin (red) in sections from tumor xenograft-bearing mice at day 3. Bars, 200 µm (top, left panel) and 50 µm (other panels). In ( F ), HMGB1 panel, solid red arrow shows the distribution of HMGB1 in the cytoplasm; solid white arrow shows the irregular nuclear morphology. Day 3: mock irradiation, n = 7; BNCT group, n = 7. Day 8: mock irradiation, n = 4; BNCT group, n = 4. Physical dose at the skin was estimated to be 6.5 Gy in the BNCT group. For counterstaining of nuclei, 4′,6-diamidino-2-phenylindole (blue) was used.

Journal: Biology

Article Title: Extracellular Release of HMGB1 as an Early Potential Biomarker for the Therapeutic Response in a Xenograft Model of Boron Neutron Capture Therapy

doi: 10.3390/biology11030420

Figure Lengend Snippet: Changes in HMGB1 levels and localization in response to neutron irradiation with BPA in SAS cell-derived tumors. SAS cells were subcutaneously grafted in nude mice and 30 min after administration of BPA–fructose at 500 mg/kg bodyweight, tumors were mock-irradiated ( E ) or locally irradiated for 60 min with neutron beam, (( F ), BNCT). ( A ) Changes in tumor volumes in response to neutron irradiation after BPA administration. Data are presented as mean ± S.E. *, p < 0.05. ( B ) Measurement of plasma levels of human HMGB1 in mice using ELISA (Abnova). ( C ) Mouse plasma HMGB1 levels of C57BL/6 mice without tumor xenograft 3 days after mock irradiation or BNCT measured using ELISA kit (Novus Biologicals). The BNCT group was administered with BPA–fructose at 500 mg/kg bodyweight 30 min before whole-body neutron irradiation. ( D ) Immunostaining of 53BP1 in sections from tumor xenografts of ( A ) at day 3. Bars in ( D ), 50 µm. ( E , F ) Immunostaining of the HMGB1 (green) and β-actin (red) in sections from tumor xenograft-bearing mice at day 3. Bars, 200 µm (top, left panel) and 50 µm (other panels). In ( F ), HMGB1 panel, solid red arrow shows the distribution of HMGB1 in the cytoplasm; solid white arrow shows the irregular nuclear morphology. Day 3: mock irradiation, n = 7; BNCT group, n = 7. Day 8: mock irradiation, n = 4; BNCT group, n = 4. Physical dose at the skin was estimated to be 6.5 Gy in the BNCT group. For counterstaining of nuclei, 4′,6-diamidino-2-phenylindole (blue) was used.

Article Snippet: These sections were washed six times with PBS and then incubated with the secondary antibody and 4’,6-diamidino-2-phenylindole (for staining of nuclei, 049-18801, Wako Pure Chemical Industries, Osaka, Japan) for 3 h in the dark at room temperature.

Techniques: Irradiation, Derivative Assay, Enzyme-linked Immunosorbent Assay, Immunostaining